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Pilot Bioreactors for Scale-Up: What’s Your Take?

by casazer on Feb 12th, 2025 09:42 AM

Hey everyone, I’m looking into pilot bioreactors (10L–200L range) for scaling up a microbial fermentation process (Pichia pastoris). I’ve been reading about SIP (Sterilizable-In-Place) systems and single-use options, but I’m curious about real-world experiences. How do these systems handle mixing, oxygen transfer, and cleaning?

casazer

Posts: 10

Joined: 15.03.2024

Re: Pilot Bioreactors for Scale-Up: What’s Your Take?

by mortexxy on Feb 12th, 2025 11:45 AM

Hey! If you're looking into pilot-scale bioreactors for your Pichia pastoris fermentation, Pilot Bioreactors https://brsbiotech.com/catalog/stainless-steel-fermentors-bioreactors/pilot-fermentors-bioreactors/be a great option. Their systems are equipped with features that ensure efficient mixing and oxygen transfer, which are crucial for scaling up microbial processes. SIP (Sterilizable-In-Place) systems are convenient for maintaining sterility without disassembling the equipment, while single-use bioreactors can save time and reduce contamination risks. Cleaning processes are streamlined with their designs, particularly when using SIP or integrated CIP options. For real-world performance, these systems handle both mixing and cleaning effectively, depending on the specific configuration you choose.

mortexxy

Posts: 9

Joined: 17.03.2024

Re: Pilot Bioreactors for Scale-Up: What’s Your Take?

by axelrz on Feb 13th, 2025 08:05 AM

I’ve used Bionet’s F2 pilot bioreactors (50L) for yeast fermentation, and they’re decent for scale-up. Mixing is good with Rushton impellers, but oxygen transfer struggled at higher cell densities—needed to add a microsparger. CIP worked fine, but steam generation (if you go for AUTOSIP) can be slow, so plan for downtime. Single-use systems are tempting, but costs add up fast, especially for larger volumes.

axelrz

Posts: 10

Joined: 17.03.2024